Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes

Authors

  • Tagreed U.Mohammd Chemistry Dept., College of Education-Ibn AL- Haytham, Baghdad University, Baghdad, Iraq Author
  • Saad S. Saleh Chemistry Dept., College of Science for Women, University of Baghdad, Baghdad, Iraq Author
  • Nadia M.Al-Mhana Chemistry Dept., College of Education-Ibn AL- Haytham, Baghdad University, Baghdad, Iraq Author
  • Azhaar F.Abd Al-Zhra College of Engineering, Al-Mustansiriyah University, Baghdad, Iraq Author

Keywords:

Aspartate aminotransferase, Purification, Diabetes

Abstract

Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and serum of patients with type 2 diabetes respectively. The purified enzyme showed single peak. The results of this study revealed that AST activity of type 2 diabetes urine and serum increased significantly (p<0.001) compared with control group.

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Key Dates

Published

2012-12-01

Issue

Vol. 16 No. 4 (2012): Journal of Engineering and Development

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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How to Cite

Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes. (2012). Journal of Engineering and Sustainable Development, 16(4), 453-461. https://jeasd.uomustansiriyah.edu.iq/index.php/jeasd/article/view/1256